Journal: The Journal of Cell Biology

Article Title: Ip-10 Inhibits Epidermal Growth Factor–Induced Motility by Decreasing Epidermal Growth Factor Receptor–Mediated Calpain Activity

doi:

Figure Lengend Snippet: Hs68 Cellular Morphometry

Article Snippet: Hs68 normal human diploid fibroblasts were purchased from American Type Culture Collection.

Techniques:

Journal: BMC Complementary and Alternative Medicine

Article Title: Protective effects and mechanisms of Terminalia catappa L. methenolic extract on hydrogen-peroxide-induced oxidative stress in human skin fibroblasts

doi: 10.1186/s12906-018-2308-4

Figure Lengend Snippet: The cytotoxicity and effect on intracellular oxidative stress in Hs68 of TCE. a Cell viability (%) of human fibroblasts (Hs68), human keratinocytes, and mouse melanoma cells treated with TCE; b Cell viability of Hs68 after treatment with TCE with or without 150 μM hydrogen peroxide (H 2 O 2 ) exposure. c Repressive effect of TCE on H 2 O 2 -induced intracellular oxidative stress in Hs68. Significant difference versus control: * p < 0.05; ** p < 0.01; *** p < 0.001. Significant inhibition versus H 2 O 2 -exposed group: # p < 0.05; ## p < 0.01

Article Snippet: Hs68, HaCaT cells, and B16F0 cells were purchased from the Bioresource Collection and Research Center in Hsinchu, Taiwan.

Techniques: Control, Inhibition

Journal: BMC Complementary and Alternative Medicine

Article Title: Protective effects and mechanisms of Terminalia catappa L. methenolic extract on hydrogen-peroxide-induced oxidative stress in human skin fibroblasts

doi: 10.1186/s12906-018-2308-4

Figure Lengend Snippet: Effect of TCE on the H 2 O 2 -induced ( a ) phosphorylation of mitogen-activated protein kinases, ( b ) c-Jun phosphorylation and c-Fos, ( c ) matrix metalloproteinase (MMP)-1, − 3, − 9, ( d ) hemeoxygenase-1 (HO-1), ( e ) cyclooxygenase-2 (COX-2) and ( f ) type I procollagen protein expressions in Hs68. Significant difference versus control: * p < 0.05; ** p < 0.01; *** p < 0.001. Significant inhibition versus H 2 O 2 -exposed group: # p < 0.05; ## p < 0.01; ### p < 0.001. EGCG: (−)-epigallocatechin gallate was used as a positive control

Article Snippet: Hs68, HaCaT cells, and B16F0 cells were purchased from the Bioresource Collection and Research Center in Hsinchu, Taiwan.

Techniques: Phospho-proteomics, Control, Inhibition, Positive Control

Journal: British Journal of Pharmacology

Article Title: WMJ‐8‐B, a novel hydroxamate derivative, induces MDA‐MB‐231 breast cancer cell death via the SHP‐1‐STAT3‐survivin cascade

doi: 10.1111/bph.13929

Figure Lengend Snippet: WMJ‐8‐B suppressed cell proliferation and affected cell cycle distribution in MDA‐MB‐231 cells. (A) Chemical structures of WMJ‐8‐A, WMJ‐8‐B, WMJ‐8‐C, WMJ‐8‐D, WMJ‐8‐E and WMJ‐8‐F. (B) 4T1, T47D, MCF‐7 and MDA‐MB‐231 cells were treated with vehicle or WMJ‐8‐A~F at 10 μM for 48 h. Cell viability was then determined by an MTT assay. Each column represents the mean ± SEM of six independent experiments performed in triplicate. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. Technical replicates were used to ensure the reliability of singe values for each experiment. (C) MDA‐MB‐231, MCF‐7, MCF‐10A and Hs68 cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. Cell viability was then determined by an MTT assay. Each column represents the mean ± SEM of six independent experiments performed in triplicate. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. (D) Cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. The acetylation status of H3 was then determined by immunoblotting. Each column represents the mean ± SEM of five independent experiments. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. (E) Cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. The percentage of PI‐stained cells in subG1, G0/G1, S and G2/M phases was then analysed by flow cytometric analysis as described in the Methods section. Each column represents the mean ± SEM of five independent experiments. Statistically significant differences were determined using one‐way ANOVA, with Tukey's post hoc test. *P < 0.05, compared with the control group.

Article Snippet: Cell culture MDA‐MB‐231, MCF‐7, T47D, 4T1 and Hs68 cell lines were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan).

Techniques: MTT Assay, Control, Western Blot, Staining

Journal: British Journal of Pharmacology

Article Title: WMJ‐8‐B, a novel hydroxamate derivative, induces MDA‐MB‐231 breast cancer cell death via the SHP‐1‐STAT3‐survivin cascade

doi: 10.1111/bph.13929

Figure Lengend Snippet: WMJ‐8‐B suppressed cell proliferation and affected cell cycle distribution in MDA‐MB‐231 cells. (A) Chemical structures of WMJ‐8‐A, WMJ‐8‐B, WMJ‐8‐C, WMJ‐8‐D, WMJ‐8‐E and WMJ‐8‐F. (B) 4T1, T47D, MCF‐7 and MDA‐MB‐231 cells were treated with vehicle or WMJ‐8‐A~F at 10 μM for 48 h. Cell viability was then determined by an MTT assay. Each column represents the mean ± SEM of six independent experiments performed in triplicate. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. Technical replicates were used to ensure the reliability of singe values for each experiment. (C) MDA‐MB‐231, MCF‐7, MCF‐10A and Hs68 cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. Cell viability was then determined by an MTT assay. Each column represents the mean ± SEM of six independent experiments performed in triplicate. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. (D) Cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. The acetylation status of H3 was then determined by immunoblotting. Each column represents the mean ± SEM of five independent experiments. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. (E) Cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. The percentage of PI‐stained cells in subG1, G0/G1, S and G2/M phases was then analysed by flow cytometric analysis as described in the Methods section. Each column represents the mean ± SEM of five independent experiments. Statistically significant differences were determined using one‐way ANOVA, with Tukey's post hoc test. *P < 0.05, compared with the control group.

Article Snippet: MDA‐MB‐231, MCF‐7, T47D, 4T1 and Hs68 cell lines were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan).

Techniques: MTT Assay, Control, Western Blot, Staining